Table 2 Primer sequences and primer conditions

From: Expression of HER3, HER4 and their ligand heregulin-4 is associated with better survival in bladder cancer patients

mRNA species (amplicon size)

Gene-specific primers (sense/antisense) (primer conc./annealing temperature)

External calibrator

HER3 365 bp

5′-GGT GCT GGG CTT GCT TTT-3′ 5′-CGT GGC TGG AGT TGG TGT TA-3′ (5 pmol/65°C)

HEC

HER4 265 bp

5′-TGT GAG AAG ATG GAA GAT GGC-3′ 5′-GTT GTG GTA AAG TGG AAT GGC-3′ (5 pmol/65°C)

KLE

HRG1α 179 bp

5′-ATC CAC CAC TGG GAC A-3′ 5′-TTT GGA TCA TGG GCA-3′ (5 pmol/60°C)

KLE

HRG1β 321 bp

5′-TAG GAA ATG ACA GTG CCT C-3′ 5′-CGT AGT TTT GGC AGC GA-3′ (5 pmol/65°C)

KLE

HRG2α 308 bp

5′-AAA TAT GGC AAC GGC AG-3′ 5′-CGC AAA GGC AGT TTC T-3′ (5 pmol/60°C)

KLE

HRG2β 236 bp

5′-GCT TTA CGT CAA CAG CG-3′ 5′-CCG GTG TAT CCC ACA G-3′ (5 pmol/63°C)

HCV

HRG3 256 bp

5′-ACA GTG CAA GCG AAA AC-3′ 5′-CAC TAT GAT ATG AGG GCG-3′ (5 pmol/61°C)

KLE

HRG4 107 bp

5′-CTGTTGTCTGCGGTATTC-3′ 5′-TCATTCTTGGTCAAGAGAGT-3′ (5 pmol/61°C)

RT4

Beta-actin 313

5′-GGCGGCACCACCATGTACCCT-3′ 5′-AGGGGCCGGACTCGTCATACT-3′ (15 pmol/68°C)

KLE