Figure 3

Adoptive transfer of MEAMNC bulk CTL induced by ACE from HER-2/neu-overexpressing HLA-A2.1⁺ primary ovarian tumour cells (Ova-1, Ova-2 and Ova-3) suppress the growth of human HER-2/neu⁺, HLA-A2.1⁺ tumour lines in SCID mice (C, D and E, respectively). (F, G) Results with MEAMNC bulk CTL induced by ACE from HER-2/neu⁻ HLA-A2.1+ primary Ova-4 and Ova-5 tumour cells, respectively. Cytotoxic T lymphocytes were i.p. injected at 2 × 10⁷ cells per injection (one injection was given per mouse) in SCID mice with s.c. growing tumours which were induced upon inoculation, 10–16 days before, with the indicated human tumour lines or the mouse fibrosarcoma MC57X. Control CTL (B) consisted of CTL specific for the melanoma gp (9₁₅₄) peptide. (A) Growth of tumour lines in untreated SCID mice. Cytotoxic T lymphocytes used for adoptive transfer were expanded (as described in Materials and Methods) from the same cultures shown in the previous figures and presented in Table 1. One experiment out of two with similar results is shown. In rapidly growing tumours, the s.d. was too low (i.e. <5% of the means) and thus omitted.