Table 7 Molecular genetic testing consumables and unit costs
Method | Components (supplier) | Cost per unit (£) |
|---|---|---|
DNA extraction | Whatman kit (Abbott Diagnostics, Maidenhead, Berkshire, UK) | £3.50 |
Polymerase chain reaction (PCR) | • 166 mM (NH4)2SO4, 670 mM Tris-HCl (pH 8.3), 37 mM M MgCl2, 850 μg/ml BSA | £0.66 |
• 0.5 μ M forward and reverse primers (synthetic DNA molecules – Invitrogen, Paisley, Scotland, UK) | ||
• 0.5 U Taq polymerase (Invitrogen, Paisley, Scotland, UK) | ||
• 750 μ M deoxyribonucleotides (Invitrogen, Paisley, Scotland, UK) | ||
• 100 ng genomic DNA | ||
Gel electrophoresis | • DGGE (9% (37.1 acyrlamide : 1 bis-acrylamide) polyacrylamide, 0–100% formamide, 1 × TAE) | £0.24 |
• DHPLC (Transgenomics, Crewe, Cheshire, UK) | ||
• Automated DNA sequencer (Applied Biosystems, Foster City, CA, USA) | ||
Sequence analysis | • ABI BigDye3.0 kit (Applied Biosystems, Foster City, CA, USA) | £3.19 |
Licence fee | • Payable for use of patented PCR technology (Roche Diagnostics, Lewes, East Sussex, UK) | £2.70 (single test) £5.60 (multiple tests) |
