Figure 5
Flow cytometric analysis of dead cells after (A) VPA or (B) TSA treatment using propidium iodide staining. SK-N-BE(2) and SH-SY5Y NB cells were treated with VPA or TSA for 72 h where after both adherent and non-adherent cells were analysed. Control cells were treated with equal amounts of solvent, that is, methanol or DMSO, respectively. Experiments were performed in triplicates ±s.d. (C, D) Assessment of cell death using annexin V staining. Cells were cultured in the presence of VPA (1 or 2 mM) or solvent for 72 h and analysed by flow cytometry. Data are presented as a representative histogram (C) or as a quantification of the cells within the M1 gate (D).
