Figure 7
Differentiation status of VPA treated SH-SY5Y and SK-N-BE(2) NB cells. (A) Real-time quantitative RT–PCR analyses of NB cells treated with 1 or 2 mM VPA for 72 h showing NPY and GAP-43 mRNA expression. Data are presented as relative expression levels using GAPDH and HPRT1 as reference genes. Experiments were performed in triplicates ±s.d. (B) Northern blot analysis of total RNA (15 μg) from SH-SY5Y and SK-N-BE(2) NB cells treated with 1 or 2 mM VPA for 72 h. Blotted filters were hybridised with a [32P]cDNA probe for NPY. (C) Real-time quantitative RT–PCR analyses of SK-N-BE(2) cells treated with 1 mM VPA in combination with 5 μ M L-685,458 for 72 h. Data are presented as relative expression level using GAPDH and HPRT1 as reference genes. Experiments were performed in triplicates ±s.d.
