Figure 2
Immunoblotting analysis of C regulators in the concentrated cell culture supernatants of Caov-3, PA-1, SK-OV-3 and SW626 ovarian cancer cells. Confluent cultures of ovarian cancer cells were incubated with serum-free cell culture medium for 48 h. The centrifuged and filtered (0.45 μm) cell culture supernatants were concentrated (100 ×). Aliquots (20 μl) were electrophoresed in 10% nonreducing SDS–PAGE slab gels and blotted onto nitrocellulose membranes. The membranes were incubated with antibodies to CD46 (J4-48), CD55 (BRIC 230 or 216), CD59 (BRIC 229) and SP40,40.
