Figure 3
Complement-mediated lysis of PA-1 and Caov-3 cells with ascitic fluids from ovarian cancer patients. Caov-3 and PA-1 cells were labelled with 51Cr. After sensitising with the anti-CD59 C-activating mAb (YTH53.1; 350 μg ml−1), the cells were exposed to ascites fluid (•) or normal human serum (○). The AF and serum dilutions are indicated on the x-axis. The released radioactivity was counted from the supernatants after a 30 min incubation at 37°C. Cell lysis was determined as a percentage of the maximum releasable radioactivity: ((released radioactivity−spontaneous release)/(maximum released radioactivity−spontaneous release)) × 100%.
