Figure 5
Immunohistochemical analysis of the C deposits on ovarian tumour cells isolated from AF. The cell specimens were stained with polyclonal antibodies against C1q (A) and C3d (B) and a mAb directed against a TCC neoantigen (C). Normal rabbit serum was used as a negative control (D). The bound Abs were detected using an immunoperoxidase staining kit, and the slides were counterstained with haematoxylin. Original magnifications, × 40 (A) and × 200 (B, C and D). Deposits of C1q (A) and C3d (B), but not of C5b-9 (C), could be detected. No reactivity was seen when the primary antibody was replaced with normal rabbit serum (D).
