Figure 6
Immunoblotting analysis of IgG in AF reacting with ovarian cancer cell membrane antigens. Cell membranes from Caov-3, PA-1, SK-OV-3 and SW626 cells were isolated and electrophoresed in 5–15% SDS–PAGE gels and transferred onto nitrocellulose filters. The nitrocellulose strips were incubated with the AF samples (diluted 1 : 800). Bound IgG was detected by the enhanced chemiluminescence system.
