Figure 3 | British Journal of Cancer

Figure 3

From: Adenoviral vector-mediated expression of a gene encoding secreted, EpCAM-targeted carboxylesterase-2 sensitises colon cancer spheroids to CPT-11

Figure 3

Cytotoxicity assay on SW1398 and Colo205 colon cancer spheroids. SW1398 (A) and Colo205 (B) spheroids were transduced with 1 × 107 PFU AdGFP or Ad.C28-sCE2. At 7 days after infection, spheroids were subjected to a range of CPT-11 concentrations and cultured for a further 7 days. Cell viability of untransduced spheroids treated with CPT-11 (closed black circles) or SN-38 (open black circles), AdGFP-transduced spheroids treated with CPT-11 (open black triangles) and Ad.C28-sCE2-transduced spheroids treated with CPT-11 (closed black squares) were analysed by WST-1 conversion measurement.

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