Table 1 Within-day precision in the measurement of XIAP by real-time RT–PCR in QC samples

From: Validation of pharmacodynamic assays to evaluate the clinical efficacy of an antisense compound (AEG 35156) targeted to the X-linked inhibitor of apoptosis protein XIAP

 

Delta CT

CT XIAP

Eight replicates in 1 day

Average

CV (%)

Average

CV (%)

GAPDH

    

 U6-E1 (high QC)

7.3

2.27

28.36

0.34

 X-G4 (low QC)

10.1

1.75

31.23

0.58

B2M

    

 U6-E1 (high QC)

4.8

4.23

28.26

0.48

 X-G4 (low QC)

7.85

3.2

31.22

0.59

Back to article page