Table 3 Within-day precision in the measurement of XIAP by Western blot analysis in QC samples

From: Validation of pharmacodynamic assays to evaluate the clinical efficacy of an antisense compound (AEG 35156) targeted to the X-linked inhibitor of apoptosis protein XIAP

 

XIAP/GAPDH ratio

XIAP minus background

Eight replicates

Mean value (pg per μ g total protein)

Precision (CV%)

Mean value (pg per μ g total protein)

Precision (CV%)

SF268 (low QC)

10.3

11.2

12.3

9.1

HeLa (high QC)

28.1

12.8

28.9

11.0

Back to article page