Table 4 Between-day precision in the measurement of XIAP by Western blot analysis in QC samples

From: Validation of pharmacodynamic assays to evaluate the clinical efficacy of an antisense compound (AEG 35156) targeted to the X-linked inhibitor of apoptosis protein XIAP

 

XIAP/GAPDH ratio

XIAP minus background

Five different days, three replicates

Mean value (pg per μ g total protein)

Precision (CV%)

Mean value (pg μ g total protein)

Precision (CV%)

SF268 (low QC)

8.0

28.7

8.6

33.5

HeLa (high QC)

30.1

22.8

34.1

23.6

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