Figure 2

Effect of in vivo single dose (15 Gy) irradiation on the ex vivo metacholine-induced Ca²⁺_imobilisation. Ca²⁺_i was calculated from the fluorescence at two excitation wavelengths (340 and 380 nm), in Fura-2-A/M-loaded isolated rat parotid gland cells (1 × 10⁶ cells ml⁻¹) in the presence of 1 mM Ca²⁺. Basal [Ca²⁺_i]: 104±7 nM for unirradiated glands, and 130±10, 103±7, and 151±17 nM at 1, 3 and 10 days after irradiation, respectively. Each point represents five or more independent measurements. Significantly different from unirradiated situation: ^*P<0.05 (Student's t-test).