Figure 5

Changes in the levels of membrane-bound PKCα following PMA stimulation of intact rat parotid gland acinar cells, 10 days after in vivo (sham-) irradiation. Isolated parotid gland cells were treated with PMA for 3 min at the indicated doses. (A) Protein kinase C alpha expression was determined by immunoblot analysis as described under ‘Materials and Methods’. 80- indicate molecular mass marker. Always 10 μg of protein was mounted (B) Diagram of at least three independent experiments. (C) Protein kinase C alpha and Na-K-ATPase expression was determined. (D) Diagram of at least three independent experiments. Significantly different from unirradiated situation: ^*P<0.05 (Student's t-test).