Figure 1

P-Ser473 Akt and Akt total standard curves for validation of P-Ser473-Akt activity ELISA assays. The following equations apply to the P-Ser473 Akt and Akt total concentrations: y=4.628x/(25.39+x), R²=0.99 (P-Ser473 Akt); and y=8.116x/(29.91+x), R²=0.99 (Akt total).