Figure 2

There is no active Wnt signalling in the fetal colon epithelial cells. TOPflash and FOPflash activities were measured in colon epithelial cells containing empty vector, WTMet or Metmut constructs. As a positive control for active Wnt signalling, TOPflash and FOPflash activities were also measured in DLD1 cells. Measurements were corrected for differences in transfection efficiency using a Renilla luciferase-encoded construct.