Figure 3

Overexpression of WT or mutated MET leads to higher phosphorylation levels of MET and its downstream targets PKB and MAPK. Fetal human colon epithelial cells were transiently transfected with empty vector, WTMet or Metmut constructs. Cells were stimulated with 100 ng ml⁻¹ HGF for 3 min and protein lysates were prepared. Blots were stained with the following antibodies: anti-MET, anti-phospho-Met, anti-phospho-PKB, anti-phospho-MAPK and as loading control, anti-PKB, anti-MAPK and anti-β-Actin.