Figure 5

Overexpression of WT or mutated MET leads to colony formation in soft agar. Fetal human colon epithelial cells were transfected with empty vector, WTMet or Metmut constructs, trypsinised and 5000 cells were used in a soft agar assay. Cells were grown in soft agar for 10 days, after which colonies were stained and counted. Increase in the amount of colonies is determined in comparison to the amount of colonies from the mock-transfected cells.