Figure 3
Specificity of tumour rejection. Two groups of BALB/c mice were immunised on days 0 and 14 by s.c. injection of Tc-ErbB2/Th-HA liposomes corresponding to 15 μg of coupled Tc-ErbB2 peptide per injection. On day 20, one group of animals was inoculated with Renca-lacZ/ErbB2 tumour cells (open circles) by s.c. injection into each flank. The other group was injected with ErbB2-negative parental Renca-lacZ cells (filled diamonds). Tumour growth was followed by caliper measurements and mean tumour volumes were calculated. Tumour-bearing mice were killed latest when tumour size reached 0.8 cm3, to avoid suffering of the animals.
