Table 4 Verification of microarray data by quantitative RT–PCR analysis

Gene	Treatment	PCR ratio ^a	Microarray ratio ^b
ATF4	Gastrin	2.1	1.6
	HGF	1.5	1.5
BTG1	Gastrin	1.2	1.5
	HGF	1.2	1.2
RRM2	Gastrin	1.2	1.7
	HGF	1.0	1.2
LAMR1	Gastrin	1.3	1.6
	HGF	1.1	1.3
IGFBPI	Gastrin	0.9	0.7
	HGF	0.8	0.8
EPS15	Gastrin	1.0	0.7
	HGF	NA	0.6
PIM1	Gastrin	1.8	0.8
	HGF	1.1	0.6

RT–PCR=reverse transcriptase–polymerase chain reaction; HGF=hepatocyte growth factor; NA; not analysed.
^aRNA transcript level in arbitrary units was standardised by the level of GAPDH, and expressed as fold change in treated relative to untreated cells. RT–PCR analysis was performed with the same total RNA as used in the microarray experiments. PCR ratios calculated from analysis of one biological sample with either gastrin or HGF are shown.
^bCorresponding microarray ratio for the same biological sample.

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