Figure 2
From: Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours
Verification of the microarray data by RT–PCR. RT–PCR primers for selected genes were used on a panel of different testicular tissue samples and cell lines. Gene-specific primers were designed preferentially to span intron–exon boundaries. The genes were divided according to the microarray analysis into: (A) Genes preferentially overexpressed in SEMs and (B) genes preferentially overexpressed in N-SEM. At the lower part of the figure, expression of ACTB is shown as a control of the RT–PCR protocol. Sample abbreviations can be found in Table 1, except for: M=100 bp marker, H2O=control, NT2=NT2 cell line days 0–15 after RA treatment, 2102Ep=2102Ep cell line days 0–10 after RA treatment.
