Figure 3

Identification of the expressing cell types by ISH. In situ hybridisation was performed with antisense and control sense (inserted images) RNA probes. Expression is shown for three genes: KIT (A–D), HLXB9 (E–H), and the IMAGE clone number 260215 (I–M). KIT was highly expressed in CIS cells (A) and in SEM (C), and present in components of teratoma, for example, keratinised intestine-like epithelium as shown here (B) and embryonal carcinoma (D). HLXB9 expression was high in CIS cells (E+F) and SEM (G); HLXB9 was present in embryonal carcinoma tumour components (H) and it was detected in spermatocytes, spermatids and Leydig cells in normal testicular tissue (E). IMAGE clone 260215 was present in spermatocytes, spermatids and Sertoli cells in normal testicular tissue (I); the expression in Sertoli cells was confirmed in a tubule with Sertoli cell-only pattern (J). Additionally, the transcript was highly expressed in CIS cells (K) and present in SEM (L) and components of embryonal carcinoma (M). Scale bar represents 50 μm.