Figure 7
KP372-1 inhibits Akt phosphorylation and some of the downstream signalling molecules as well as Akt kinase activity. (A) NPA187 and WRO cells were treated with the IC50 concentrations of KP372-1 (30–60 nM, respectively) for 4 h in RPMI medium without serum. Equal amounts of protein were resolved by SDS–polyacrylamide gel electrophoresis and immunoblotted with different antibodies as indicated. (B) KP372-1 inhibits Akt kinase activity. Different thyroid cancer cells were treated with KP372-1 for 2 h, cell lysates were prepared, and Akt was immunoprecipitated and analysed for Akt-Ser473 and Akt kinase activity using an in vitro kinase assay with GSK-β as a substrate. Results shown are representative of three experiments.
