Figure 1

Custom made oligonucleotide array design. 70-mer oligo's, corresponding to 298 genes that were previously found to be differentially expressed in cell lines 1F6 and Mel57 and four additional housekeeping genes, were spotted in octaplicate on UltraGAPS slides. The housekeeping genes were randomly localised throughout the array. Additionally, various other controls for labelling and hybridisation efficiency were present on the array, such as oligo's of non-human species, Cot-1 repetitive sequences, polyA sequences and spots containing only spotting buffer.