Figure 3
Analysis of protein expression of THBD, GDF15 and FABP7 in human melanoma cell lines 1F6 and Mel57. Magnification (× 400) of AgarCyto's (A–H) and cultured 1F6 and Mel57 cells (I, J). (A, B) Negative control of 1F6 and Mel57, respectively, leaving out specific antisera. (C, D, I, J) IHC using THBD specific antibodies; in AgarCyto's as well as cultured cells most 1F6 cells showed a (membranous) THBD specific staining (C, I), whereas Mel57 cells were completely negative (D, J). (E, F) IHC using GDF15 specific antibodies; a more intense cytoplasmic GDF15 staining could be detected in cell line Mel57 (F), compared to 1F6 cells (E). (G, H) IHC using FABP7 specific antibodies; 1F6 cells showed a higher immunoreactivity for FABP7 (cytoplasmic) (G) than Mel57 cells (H). (K) Western blot analysis of GDF15; a distinct preferential protein expression could be detected in Mel57 compared to 1F6. The 35 kDa band represents the pro-form of GDF15 (pro-peptide+mature protein), whereas the 25 kDa band shows the cleaved GDF15 pro-peptide.
