Figure 4
Verification of differential gene expression in human melanoctyic tumour progression lesions by semiquantitative RT–PCR. (A) For 12 differentially expressed genes, relative expression to GAPDH was determined by semiquantitative RT–PCR as shown for FABP7. (B) For MYL6 and C7orf20 differential expression could hardly be detected. THBD, FABP7, H2AFJ, RRAGD, MYADM and HR showed a downregulated expression during melanoma progression, with a preferable decrease in MM lesions. The latter was especially true for H2AFJ. Expression of CKS2, NCK2 and GDF15 showed an upregulation associated with increased malignancy of melanocytic tumour lesions. For THBD, FABP7, H2AFJ, RRAGD, MYADM, HR, CKS2, NCK2 and GDF15 differential expression found by semiquantitative RT–PCR was in accordance with our custom array data, whereas CTSL showed an inverse differential expression.
