Figure 3
Phospho-protein analysis in additional RCC cell lines. Six RCC cell lines were grown to 50% confluency, then treated for 2 h with DMSO (lanes 1, 5, 9, 13, 17 and 21), rapamycin (10 nM; lanes 2, 6, 10, 14, 18 and 22), Iressa (10 μ M; lanes 3, 7, 11, 15, 19 and 23) or both (lanes 4, 8, 12, 16, 20 and 24). Lysates were analysed as in Figure 1. VHL mutational status is indicated along the bottom.
