Figure 5
Polysome analysis of WT8 cells. WT8 cells were grown to 70% confluency, then treated for 2 h with DMSO, rapamycin (10 nM), Iressa (10 μ M) or both, and harvested for polysome fractionation. A representative A280 fractionation profile is shown along with the fractions pooled and the results from quantitative RT–PCR analysis.
