Figure 3
Standard curve for methylene blue staining against TS cell number. To ensure methylene blue staining was representative of the total number of cells present in a 3D TS, the assay was performed on TS of defined cell number from 1 × 103 to 3 × 104 cells. Staining, solubilisation and absorbance measurements were carried out and the standard curve of absorbance against TS cell number was plotted.
