Figure 5 | British Journal of Cancer

Figure 5

From: Adenovirus-mediated interferon α gene transfer induces regional direct cytotoxicity and possible systemic immunity against pancreatic cancer

Figure 5

Expression of NKG2D ligands on pancreatic cancer cell lines. (A) RT–PCR analyses of NKG2D ligands. Total RNA was extracted from cell lines and after reverse transcription the mRNA of MICA, MICB, ULBP-1, ULBP-2 and ULBP-3 was detected by PCR. (B) Flow cytometric analysis of MICA and MICB. Cells were incubated with anti-human MICA/B monoclonal antibody (filled curve) or isotype control antibody (open curve), and flow cytometry was carried out using a FACScan system.

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