Figure 2

(A) FACScan analysis for cell surface Fas in T24 and RT4 cell lines. (B) Immunoblotting for FasL and FLIP in UC cell lines and normal urothelium compared with two control cell lines; SW480 to FasL and HEK 293T cells transfected with human FLIP. A single band of 37 kDa for FasL and 58 kDa for FLIP were detected. β-Actin was applied as a control for loading. (C) Immunoblotting for FasL, caspase-8 and Bid in T24 and RT4 cell lines following ADM treatment.