Table 2 Primers sequences for RT–PCR and real-time PCR
Specificity forward (5′ → 3′) | Reverse (5′ → 3′) | Product size (bps) | Annealing T. (°C) |
|---|---|---|---|
RT–PCR | |||
Fas atgctgggcatctggaccct | gccatgtccttcatcacacaa | 335 | 58 |
FasL aactcaaggtccatgcctctg | ggtgagttgaggagctacagaca | 553 | 58 |
DcR3 gccactacacgcagttctggaacta | gaggaagagcctggcacattgaggg | 336 | 60 |
DR4 attgtacgccctggagtgac | aaggacacggcagagcctgtgccat | 741 | 60 |
DR5 ctgaaaggcatctgctcaggtg | cagagtctgcattaccttctag | 582 | 58 |
DcR1 gccggaagtgtagcaggtg | ggggcaggggcaggcgtttct | 546 | 58 |
DcR2 cttttccggcggcgttcatgtccttc | gtttcttccaggctgcttccctttgtag | 463 | 58 |
β-ACT aatgcttctaggcggact | actcccagggagaccaaa | 533 | 58 |
Real-time PCR | |||
DcR3 cttcttcgcgcacgctc | atcacgccggcaccag |
