Figure 4
(A) Proliferative responses of Th clones from patient 19. In all, 72 T-cell clones were generated from postvaccine T cells, of these 10 were specific for transfected DC. All were of the CD4+ phenotype. Seven representative positive clones are shown here. Results are given as mean c.p.m. of triplicate wells with standard error of the mean (s.e.m.) indicated on the bars. (B) Killing of 51Cr target cells by CTL clones derived from patient 10. Two of CD8+ clones were derived from P10, who shared HLA-A9 with the tumour cell line PC-3. Results are given as specific lysis of target cells. The prostate cancer cell line PC-3 and mRNA-transfected DCs are killed, but no lysis when HLA-matched EB cell lines were used as targets. The ratio of effector: target in this experiment was 25 : 1. The results are recorded as mean lysis of triplicate with s.e.m. indicated on the bars.
