Figure 5
Immunohistochemical detection of hERG1 protein in gliomas and normal brain tissue. Sample preparation, antibodies used and IHC procedures are reported in Materials and Methods. (A) Immunohistochemistry with anti-hERG1 antibodies in a GBM; (B) IHC with anti-hERG1 antibodies in an OD; note the lack of staining in neoplastic oligodendrocytes compared to the positive staining of neurons entrapped in the neoplastic lesion; (C) IHC of a normal brain sample using anti-hERG1 antibodies; c′=GFAP staining (note the positive astrocytes); c″=NF staining (note the positive neurons). Bar=50 μm.
