Figure 1
From: DNA replication licensing and cell cycle kinetics of normal and neoplastic breast
(A) Photomicrographs of paraffin-embedded tissue sections of normal premenopausal breast immunohistochemically stained with antibodies against Ki67, Mcm2 and geminin (original magnification, × 400). A high proportion of epithelial cells of the terminal duct lobular unit express Mcm2, a subpopulation Ki67 and only a minority geminin. The higher labelling index for Mcm2 compared to Ki67 reflects an additional replication licensed but nonproliferating growth fraction, identified by Mcm2 but not the standard proliferation marker Ki67. (B) Ki67, Mcm2 and geminin labelling indices for individual cases of normal premenopausal breast. Case 1 showing high levels of Ki67, Mcm2 and geminin expression is a patient who had a prophylactic mastectomy for BRCA1. All other cases of reduction mammoplasty show higher expression of Mcm2 compared to Ki67 and geminin.
