Figure 2

Effect of U0126 on extracellular Ca²⁺-mediated changes in CBS cell growth and differentiation. Upper panel: morphological appearance of CBS cells grown for 3 days in Ca²⁺-free medium with or without 10 μ M U0126 (upper left and upper right respectively) or in medium supplemented with 1.4 mM Ca²⁺ with or without 10 μ M U0126 (lower left and lower right). Lower panel: CBS cell proliferation under the same conditions. Values shown are means and s.d. based on triplicate samples in a single experiment. Statistical significance was determined by ANOVA, followed by paired group comparisons. ^* indicates differences from high-Ca²⁺ group (1.4 mM Ca²⁺ supplementation) at the P<0.05 level. The study was conducted five times with consistent results.