Figure 3

Effect of extracellular Ca²⁺ and U0126 on E-cadherin expression. Upper panel: E-cadherin expression by immunostaining of CBS cells grown for three days in nonsupplemented medium (left), in medium supplemented with 1.4 mM Ca²⁺ (centre) and in medium supplemented with 1.4 mM Ca²⁺ and 10 μ M U0126 (right). Lower panel: E-cadherin expression by Western blotting (same conditions). Quantitative values are based on densitometry scanning of bands in four separate experiments and are means and s.d. Statistical significance was determined by ANOVA, followed by paired group comparisons. ^*indicates differences from high-Ca²⁺ group (1.4 mM Ca²⁺ supplementation) at the P<0.05 level.