Figure 7
Effect of extracellular Ca2+ on E-Cadherin, CaSR, and phosphorylated-ERK expression in Ca2+-responsive and nonresponsive CBS clones. Upper panel: E-cadherin expression; Middle panel: CaSR expression; Lower panel: ERK expression. R1 and NR1 isolates of CBS cells were grown for three days in Ca2+-free medium (left) and in medium supplemented with 1.4 mM Ca2+ (right). Western blotting was used to assess the proteins. Quantitative values are based on densitometry scanning of bands in three separate experiments and are means and s.d. Statistical significance was determined by Student's t-test, comparing nonsupplemented and Ca2+-supplemented conditions for the two isolates separately. *indicates differences from the nonsupplemented medium at the P<0.05 level.
