Figure 6
Role of TACE/ADAM17 in the basal and regulated cleavage of carbonic anhydrase IX (CA IX) ECD. CHO-wild type (WT) cells, their mutated variant CHO-M2 defective in TACE/ADAM17 maturation, CHO-M2+TACE transfectants containing a fully functional TACE and CHO-Ka13 cells lacking HIF-1α were transiently transfected to express CA IX. (A) The transfected cells were allowed to shed CA IX into the medium over 24-h incubation period under normoxia and hypoxia (2% O2). The same procedure was applied to mock-transfected cells. Media and extract collected from all dishes were assayed by ELISA. The results were expressed as average absorbance values with standard deviations. (B) The transfected cells were treated for 2 h either with PMA or with pervanadate in the absence and presence of BB-94 metalloprotease inhibitor added 30 min before the treatment. The results were illustrated as a ratio between the absorbance data obtained from the induced vs control samples. *P<0.01 and **P<0.001 by Student's t-test.
