Table 1 List of the primers used for RT–PCR analysis of the metalloprotease expression in the human tumour cell lines
Sense primer | Antisense primer | |||
|---|---|---|---|---|
mRNA (GenBank accession no.) | Position | Sequence (5′ → 3′) | Position | Sequence (5′ → 3′) |
MMP1 (NM_002421) | 1293–1314 | gatccaggttatcccaaaatga | 1538–1517 | caggaaaacaccttctttggac |
MMP2 (NM_004530) | 1002–1023 | tgttcaatggcaaggagtacaa | 1358–1339 | ccaggaaagtgaaggggaag |
MMP3 (NM_002422) | 213–236 | gtttgttaggagaaaggacagtgg | 539–516 | atatcagcctctccttcatacagc |
MMP7 (BC003635) | 196–217 | agaagccaaactcaaggagatg | 411–390 | cgatccactgtaatatgcggta |
MMP9 (NM_004994) | 896–917 | atcttccaaggccaatcctact | 1082–1063 | ccaggaaagtgaaggggaag |
TIMP1 (BC007097) | 11–32 | gcgtggacatttatcctctagc | 263–242 | aaggtggtctggttgacttctg |
TIMP2 (NM_003255) | 2318–2339 | atgggggttaggataggaagaa | 2517–2496 | ccgctgaatagaacaggctaag |
MT1-MMP (X83535) | 1249–1270 | gcaaattcgtcttcttcaaagg | 1476–1455 | tgttcttggggtactcgctatc |
TACE (NM_003183) | 1612–1633 | tggatgaaggagaagagtgtga | 1874–1852 | aagatccaagcaaacagtgtcat |
β-Actin (NM_001101) | 414–433 | ccaaccgcgagaagatgacc | 649–629 | gatcttcatgaggtagtcagt |
