Figure 2
From: Elevated phosphorylation and activation of PDK-1/AKT pathway in human breast cancer
Gross view of IHC staining on TMA slides fixed with normal breast tissues and invasive breast tumours. Representative examples of IHC stained TMA slides to detect phosphorylated molecules as described in the legend to Figure 1. Nuclear staining with haematoxylin was omitted and hence red outcome indicates phosphorylated proteins in breast tissues. Noticeably, the normal breast tissues, arrayed in the left-hand row and labeled with a black arrow, revealed poor phosphorylation and remained colourless upon IHC staining. The most bottom spot (black square) of the normal tissue row was derived from placenta, which served as examples of positive staining for phosphorylation of PDK-1 (S241) and AKT (T308). The spot right above the placenta tissue was a paraffin control, which contained no tissue, whereas the remaining spots contained normal breast tissue in duplicates.
