Figure 1
From: The Wnt antagonist sFRP1 is downregulated in premalignant large bowel adenomas
Real-time PCR quantitation of sFRP1 expression levels in a normal cell line following treatment with LiCl. (A) Real-time PCR quantitation of relative sFRP1 mRNA expression measured across a series of time points following lithium chloride treatment of the normal cell line FHS 74 Int according to the comparative CT method. The initial step in the calculations is the normalisation of the sFRP1 gene to the KRT8 gene in order to normalise quantity and quality of the cDNA samples. The level of sFRP1 expression at each time point was then normalised to the level of sFRP1 expression before treatment. sFRP1 mRNA levels increased upon LiCl treatment peaking with an eight-fold increase at 8 h, which fell to six-fold at 16 h. (B) RT–PCR results for hNKD1 and β-actin at time 0 and 16 h.
