Figure 2 | British Journal of Cancer

Figure 2

From: Promoter methylation of P16, RARβ, E-cadherin, cyclin A1 and cytoglobin in oral cancer: quantitative evaluation using pyrosequencing

Figure 2

Representative pyrograms for RARβ. The four targeted cytosines are enclosed in unshaded squares (as reverse strand was read, G peaks (arrowed) indicate methylated cytosine while (A) indicates unmethylated cytosine). The control, non-CpG cytosine residue showing complete conversion of cytosine to uracil by bisulphite treatment is shown in the left-hand shaded box. Normal tissue (top panel) demonstrates no methylation while tumour tissue (bottom panel) demonstrates a significant level of methylation at all four target bases. The Methylation Index (MtI) is calculated as the average rate of G incorporation at each CpG.

Back to article page