Table 2 Methylation data and analysis

From: Promoter methylation of P16, RARβ, E-cadherin, cyclin A1 and cytoglobin in oral cancer: quantitative evaluation using pyrosequencing

Gene

Samples

% samples MtI>0 a

% samples MtI>5% b

Kruskall–Wallis test of mean rank (diff. T/N)

Reference MtI c

% tumours Reference MtI

P16

T

45% (36/80)

28% (22/80)

P=0.048

0.046

22/80 28%

 

N

27% (7/26)

4% (1/26)

   

RARβ

T

85% (68/80)

73% (58/80)

P=0.088 (NS)

0.215

15/80 19%

 

N

71% (15/21)

62% (13/21)

   

CYGB

T

74% (59/80)

65% (52/80)

P=0.002

0.149

35/80 44%

 

N

57% (12/21)

52% (11/21)

   

CYCA1

T

72% (56/78)

53% (42/78)

P=0.001

0.002

38/78 49%

 

N

11% (1/9)

0% (0/9)

   

ECAD

T

83% (59/71)

42% (30/71)

P=0.347 (NS)

0.218

1/71 1%

 

N

56% (10/18)

33% (6/18)

   
  1. aMtI=Methylation index (mean of % methylation at all CpGs/100).
  2. b0–5% methylation is considered to be due to experimental background.
  3. cMtI below which 95% of normal sample data fall.