Figure 2
Detection of endostatin by Western blot analysis. The sera of 12 patients evaluated for endostatin concentration by ELISA were separated on polyacrylamide gel and incubated with a specific endostatin antibody as described in Materials and Methods. Results of four patients are shown. Marker bands (10, 15, 25, 30 and 35 kDa) are depicted. No specific signal could be detected in the sera with 5.7 and 11.7 ng ml−1 of the inhibitor.
