Figure 6

The effect of Net1 knockdown on gastric adenocarcinoma cell proliferation and invasion. (A) Real-time PCR was used to confirm the significant siRNA-mediated reduction in Net1 expression. Using two siRNA duplexes; Net1(1) and Net1(2), Net 1 expression was reduced by 49 and 41%, respectively, in comparison with control cells. Using a combination of both siRNA duplexes; Net1(1+2) resulted in 55% decreased Net1 expression (P<0.05). (B) The proliferation of cells treated with both duplexes was compared with control cells using an MTS assay. siRNA mediated Net1 downregulation resulted in 47 and 41% decrease in cell proliferation. Using both Net1 siRNA duplexes in combination resulted in 58% decreased cell proliferation (P<0.05). (C) Downregulation of Net1 expression using both siRNA duplexes resulted in 100 and 96% reduction in cell invasion in comparison with control cells. A combination of both siRNA molecules resulted in 96% AGS cell invasion ^*(P<0.05).