Figure 7
From: Net1 and Myeov: computationally identified mediators of gastric cancer
The effect of siRNA-mediated Myeov knockdown on AGS cell proliferation and invasion. (A) Confirmation of Myeov downregulation using real-time PCR. Myeov downregulation, using two separate siRNA duplexes, Myeov(1) and Myeov(2), resulted in 100 and 30% decreased expression when compared with control cells. A combination of both Myeov siRNA molecules resulted in 75% decreased Myeov expression (P<0.05). (B) Using siRNA duplexes, cell proliferation was reduced 68 and 36%, respectively, when compared with control cells. Using both duplexes in combination resulted in 73% decrease in gastric cancer cell proliferation (P<0.05). (C) Similarly, cell invasion was reduced by 85 and 90% and by 99% using a combination of both siRNA duplexes in comparison with control cells *(P<0.05).
