Figure 2
Type I collagen promotes maximal pancreatic cancer cell proliferation. The 96-well polystyrene culture dishes, not treated for tissue culture, were coated with type I collagen, type IV collagen, fibronectin, laminin, or vitronectin as described in Materials and Methods. Twenty-four hour, serum-starved MiaPaCa-2, AsPC-1, BxPC-3, CFPAC, and FG cells (5 × 103 well−1) were cultured under serum-free conditions on the indicated ECM substrates over a 4-day time course. At the indicated time points, triplicate determinations were quantified for each cell line on each substrate by measuring the absorbance at 450 nm and subtracting the value obtained at initial seeding (T=0) using CellTiter 96 Aqueous One Solution Cell Proliferation Assay™ reagent according to the manufacturer's instructions (Promega, Madison, WI, USA). Data presented represent the mean±s.e.m. from two independent experiments.
