Figure 4
Analysis of RET/PTC rearrangements on control cell lines. (A) interphase FISH with YAC DNA probes on TPC-1 (left), SHSY (middle) and NPA (right) cell lines. All interphase cell nuclei of TPC-1 exhibit a split FISH signal and an overlapping FISH signal suggesting RET/PTC rearrangement, whereas exclusively overlapping FISH signals are visible in SHSY and NPA cells (B) RT–PCR for RET/PTC1 (left), TK domain (middle) and extracellular domain (right). RET/PTC1 rearrangement is only detectable in TPC-1 cells, whereas expression of the RET TK domain is observed in both TPC-1 and SHSY cells. Extracellular domain expression is only observed in SHSY cells, which express the full-length c-RET transcript. TPC-1 is positive for RET/PTC1 (positive control), SHSY shows expression of full-length RET without rearrangement (negative control) and the triploid NPA cell line exhibits neither RET expression nor RET rearrangement by FISH (negative control).
